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The complete nutrient medium was comprised of phenol red-containing Dulbeccos Modified Eagles medium (DMEM, Lonza) with 4.5 g/L glucose, L-glutamine and supplemented with fetal bovine serum (FBS, Sigma-Aldrich) to a 10% final concentration and penicillin/streptomycin mixture to final concentrations of 100 U/mL for each of them